{"id":2361,"date":"2019-11-21T13:43:06","date_gmt":"2019-11-21T05:43:06","guid":{"rendered":"https:\/\/www.merieuxnutrisciences.com.cn\/?p=2361"},"modified":"2023-03-09T14:33:22","modified_gmt":"2023-03-09T06:33:22","slug":"determination-of-paraquat-in-tea-uplc","status":"publish","type":"post","link":"https:\/\/www.merieuxnutrisciences.com.cn\/?p=2361","title":{"rendered":"\u8d85\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff0d\u4e32\u8054\u8d28\u8c31\u6cd5\u6d4b\u5b9a\u8336\u53f6\u4e2d\u767e\u8349\u67af"},"content":{"rendered":"
\u519c\u836f\u6b8b\u7559\u68c0\u6d4b<\/a>, <\/span>\u6885\u91cc\u57c3\u68c0\u6d4b<\/a>, <\/span>\u6885\u91cc\u57c3\u8425\u517b\u79d1\u5b66<\/a>, <\/span>\u767e\u8349\u67af\u68c0\u6d4b<\/a>, <\/span>\u8336\u53f6\u68c0\u6d4b<\/a><\/div>\n\n

\u767e\u8349\u67af\u662f\u4e00\u79cd\u901f\u6548\u63a5\u89e6\u578b\u706d\u751f\u6027\u9664\u8349\u5242\uff0c\u5c5e\u4e8e\u5b63\u94f5\u76d0\u7c7b\u9664\u8349\u5242\uff0c\u4f7f\u7528\u904d\u53ca\u5168\u4e16\u754c130\u591a\u4e2a\u56fd\u5bb6\u548c\u5730\u533a\uff3b1\uff3d<\/sup>\u3002\u767e\u8349\u67af\u6781\u6613\u6eb6\u4e8e\u6c34\uff0c\u5fae\u6eb6\u4e8e\u4f4e\u76f8\u5bf9\u5206\u5b50\u8d28\u91cf\u7684\u9187\u7c7b\uff0c\u4e0d\u6eb6\u4e8e\u70c3\u7c7b\u6eb6\u5242\uff0c\u5728\u9178\u6027\u3001\u4e2d\u6027\u6eb6\u6db2\u4e2d\u7a33\u5b9a\uff0c\u5728\u78b1\u6027\u6eb6\u6db2\u4e2d\u6613\u5206\u89e3\u3002\u968f\u7740\u767e\u8349\u67af\u7684\u65e5\u76ca\u63a8\u5e7f\u4f7f\u7528\uff0c\u5176\u5bf9\u4eba\u4f53\u5065\u5eb7\u548c\u751f\u6001\u73af\u5883\u5b58\u5728\u7684\u4e25\u91cd\u5371\u5bb3\u5728\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u5df2\u6210\u4e3a\u793e\u4f1a\u95ee\u9898\u3002\u767e\u8349\u67af\u5bf9\u4eba\u3001\u755c\u5747\u6709\u8f83\u5f3a\u7684\u6bd2\u6027\uff3b2\uff3d<\/sup>\uff0c\u76ee\u524d\u5c1a\u65e0\u7279\u6548\u6cbb\u7597\u65b9\u6cd5\u3002\u767e\u8349\u67af\u7684\u9ad8\u6bd2\u6027\u548c\u5e7f\u6cdb\u5e94\u7528\u6027\uff0c\u4f7f\u5f97\u5efa\u7acb\u5feb\u901f\u3001\u9ad8\u9009\u62e9\u6027\u3001\u9ad8\u7075\u654f\u5ea6\u7684\u767e\u8349\u67af\u68c0\u6d4b\u65b9\u6cd5\u975e\u5e38\u5fc5\u8981\u3002\u68c0\u6d4b\u7684\u65b9\u6cd5\u4e3b\u8981\u6709\u6c14\u76f8\u8272\u8c31\u6cd5\u6216\u6c14\u76f8\u8272\u8c31\uff0d\u8d28\u8c31\u6cd5\uff3b3\uff0d4\uff3d<\/sup>\u3001\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u6cd5\u6216\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff0d\u4e32\u8054\u8d28\u8c31\u6cd5\uff3b5\uff0d11\uff3d<\/sup>\u3002<\/span><\/p>\n

\u767e\u8349\u67af\u4e3a\u5f3a\u6781\u6027\u5316\u5408\u7269\uff0c\u91c7\u7528\u6c34\u6d78\u6cd5\u63d0\u53d6\u6548\u7387\u8f83\u9ad8\uff0c\u4e00\u822c\u5bf9\u6c34\u63d0\u53d6\u6db2\u51c0\u5316\u540e\u76f4\u63a5\u8fdb\u6837\u5206\u6790\uff0c\u8fd9\u6837\u65e0\u6cd5\u6ee1\u8db3\u4f4e\u542b\u91cf\u6b8b\u7559\u7684\u9700\u6c42\u3002\u82e5\u91c7\u7528\u9ad8\u6e29\u716e\u6cb8\u6cd5\u8fdb\u884c\u6d53\u7f29\uff0c\u65b9\u6cd5\u56de\u6536\u7387\u4f4e\uff0c\u524d\u5904\u7406\u64cd\u4f5c\u8d39\u65f6\u3002\u672c\u5de5\u4f5c\u91c7\u7528\u5f3a\u9178\u6027\u9633\u79bb\u5b50\u4ea4\u6362\u6811\u8102\u8fdb\u884c\u5438\u9644\uff0c\u6c2f\u5316\u94f5\u6eb6\u6db2\u8fdb\u884c\u6d17\u8131\uff0c\u518d\u5728\u78b1\u6027\u6761\u4ef6\u4e0b\u8fdb\u884c\u884d\u751f\u53cd\u5e94\uff0c\u53cd\u5e94\u4ea7\u7269\u5728C18\u8272\u8c31\u67f1\u4e0a\u5206\u79bb\u540e\uff0c\u901a\u8fc7\u4e32\u8054\u8d28\u8c31\u8fdb\u884c\u68c0\u6d4b\uff0c\u80fd\u591f\u83b7\u5f97\u51c6\u786e\u3001\u5feb\u901f\u548c\u9ad8\u7075\u654f\u5ea6\u7684\u7ed3\u679c\uff0c\u5e76\u6ee1\u8db3\u5404\u9879\u8d28\u63a7\u8981\u6c42\u3002<\/span><\/p>\n

1 \u8bd5\u9a8c\u90e8\u5206<\/strong><\/span><\/span><\/p>\n

1.1 \u4eea\u5668\u4e0e\u8bd5\u5242<\/strong><\/span><\/p>\n

WatersXevo TQ-S\u578b\u8d85\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff0d\u4e32\u8054\u8d28\u8c31\u4eea\uff1bSUPELCO VISIPREP 24TM<\/sup>DL\u578b\u56fa\u76f8\u8403\u53d6\u88c5\u7f6e\uff1bThermo Multifuge X3R\u578b\u9ad8\u901f\u79bb\u5fc3\u673a\uff1bEYELASB-1100\u578b\u65cb\u8f6c\u84b8\u53d1\u4eea\uff1bNM100-SSCX\u5f3a\u9178\u6027\u9633\u79bb\u5b50\u4ea4\u6362\u6811\u8102\uff080.15\uff5e0.25\uff4d\uff4d\uff09\u3002
\u767e\u8349\u67af\u6807\u51c6\u50a8\u5907\u6eb6\u6db2\uff1a1.00\uff47\u00b7\uff2c\uff0d1<\/sup>\uff0c\u79f0\u53d6\u767e\u8349\u67af\u6807\u51c6\u54c1\uff08\u7eaf\u5ea6\u4e0d\u5c0f\u4e8e99.0\uff05\uff090.010\uff47\uff0c\u7528\u7532\u9187\u6eb6\u89e3\u5e76\u5b9a\u5bb9\u81f310\uff4d\uff2c\uff0c\u4e8e\uff0d20\u2103\u51b7\u51bb\u4fdd\u5b58\u3002
\u767e\u8349\u67af\u6807\u51c6\u6eb6\u6db2\uff1a2.0\uff4d\uff47\u00b7\uff2c\uff0d\uff11\uff0c\u79fb\u53d61.00\uff47\u00b7\uff2c\uff0d1<\/sup>\u767e\u8349\u67af\u6807\u51c6\u50a8\u5907\u6eb6\u6db220\u03bc\uff2c\u81f310\uff4d\uff2c\u5bb9\u91cf\u74f6\u4e2d\uff0c\u7528\u6c34\u5b9a\u5bb9\u81f310\uff4d\uff2c\uff0c\u4e8e4\u2103\u51b7\u85cf\u4fdd\u5b58\u3002
\u7532\u9187\u3001\u4e59\u8148\u5747\u4e3a\u8272\u8c31\u7eaf\uff0c\u7532\u9178\u7684\u7eaf\u5ea6\u4e0d\u5c0f\u4e8e95\uff05\uff0c\u5176\u4ed6\u8bd5\u5242\u5747\u4e3a\u5206\u6790\u7eaf\uff0c\u8bd5\u9a8c\u7528\u6c34\u4e3a\u8d85\u7eaf\u6c34\uff08\u7535\u963b\u7387\u4e3a18.25\uff2d\u03a9\u00b7c\uff4d\uff09\u3002<\/span><\/p>\n

1.2 \u4eea\u5668\u5de5\u4f5c\u6761\u4ef6<\/strong><\/span><\/p>\n

1\uff09\u8272\u8c31\u6761\u4ef6Waters BEH UPLC C18<\/sub>\u00a0\u8272\u8c31\u67f1\uff082.1\uff4d\uff4d\u00d750\uff4d\uff4d\uff0c1.7\u03bc\uff4d\uff09\uff0c\u67f1\u6e2940 \u2103\uff1b\u6d41\u91cf\u4e3a0.4\uff4d\uff2c\u00b7\uff4d\uff49\uff4e\uff0d1<\/sup>\uff1b\u8fdb\u6837\u91cf\uff13\u03bc\uff2c\u3002\u6d41\u52a8\u76f8\uff1a\uff21 \u4e3a\u4e59\u8148\uff0c\uff22\u4e3a\u4e59\u8148\uff085\uff0b95\uff09\u6eb6\u6db2\uff08\u542b\u4f53\u79ef\u5206\u6570\u4e3a0.1\uff05\u7684\u7532\u9178\u548c1\u00a0\uff4d\uff4d\uff4fl\u00b7\uff2c\uff0d1<\/sup> \u4e59\u9178\u94f5\uff09\u3002\u68af\u5ea6\u6d17\u8131\u7a0b\u5e8f\uff1a0\uff5e1.0\uff4d\uff49\uff4e\u65f6\uff0c\uff21 \u4e3a\uff10\uff1b1.0\uff5e1.5\uff4d\uff49\uff4e\u65f6\uff0c\uff21 \u7531\uff10\u5347\u81f390\uff05\uff0c\u4fdd\u63011.3\uff4d\uff49\uff4e\uff1b2.8\uff5e2.9\uff4d\uff49\uff4e\u65f6\uff0c\uff21\u753190\uff05\u964d\u81f3\uff10\uff0c\u4fdd\u63011.1\uff4d\uff49\uff4e\u3002
\uff12\uff09\u8d28\u8c31\u6761\u4ef6 \u7535\u55b7\u96fe\u79bb\u5b50\u6e90\uff08ESI\uff09\uff0c\u6b63\u79bb\u5b50\u7535\u79bb\u6a21\u5f0f\uff1b\u6bdb\u7ec6\u7ba1\u7535\u538b3.0kV\uff1b\u79bb\u5b50\u6e90\u6e29\u5ea6150\u2103\uff1b\u9525\u5b54\u53cd\u5439\u6c14\u6d41\u91cf60\uff2c\u00b7\uff48\uff0d1<\/sup>\uff1b\u8131\u6eb6\u5242\u6c14\u6e29\u5ea6500\u2103\uff0c\u8131\u6eb6\u5242\u6c14\u6d41\u91cf1000\uff2c\u00b7\uff48\uff0d1<\/sup>\uff1b\u78b0\u649e\u6c60\u538b\u529b0.3\uff30\uff41\uff1b\u9525\u5b54\u7535\u538b66\uff36\uff0c\u78b0\u649e\u7535\u538b26\uff36\uff1b\u626b\u63cf\u65b9\u5f0f\u4e3a\u591a\u53cd\u5e94\u76d1\u6d4b\uff08\uff2d\uff32\uff2d\uff09\u6a21\u5f0f\uff0c\u5176\u4f59\u8d28\u8c31\u53c2\u6570\u89c1\u8868\uff11\uff0c\u5176\u4e2d\u201c\uff0a\u201d\u4e3a\u5b9a\u91cf\u79bb\u5b50\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

1.3 \u8bd5\u9a8c\u65b9\u6cd5<\/strong><\/span><\/p>\n

1.3.1 \u6837\u54c1\u63d0\u53d6<\/span><\/p>\n

\u79f0\u53d6\u5747\u8d28\u8336\u53f6\u6837\u54c1\uff082\u00b10.02\uff09\uff47\u81f350\uff4d\uff2c\u4e00\u6b21\u6027\u79bb\u5fc3\u7ba1\u4e2d\u3002\u52a0\u6c3413\uff4d\uff2c\uff0c\u4f7f\u8336\u53f6\u5145\u5206\u6da6\u6e7f\uff0c\u52a0\u5165\u786b\u91787\uff4d\uff2c\uff0c\u6da1\u65cb\u6df7\u5300\uff0c\u4e8e80\u2103\u6c34\u6d74\u4e2d\u52a0\u70ed12\uff48\u4ee5\u4e0a\u3002\u53d6\u51fa\uff0c\u51b7\u5374\u81f3\u5ba4\u6e29\u540e\uff0c\u7528\u6c34\u5b9a\u5bb9\u81f350\uff4d\uff2c\uff0c\u4ee5\u8f6c\u901f3500\uff52\u00b7min\uff0d\uff11<\/sup>\u79bb\u5fc35min\uff0c\u5f85\u7528\u3002<\/span><\/p>\n

1.3.2 \u6837\u54c1\u51c0\u5316<\/span><\/p>\n

NM100-SSCX\u5f3a\u9178\u6027\u9633\u79bb\u5b50\u4ea4\u6362\u6811\u8102\u4f7f\u7528\u524d\u9700\u4f9d\u6b21\u75283\u500d\u4f53\u79ef\u7684\u7532\u9187\u548c3\u500d\u4f53\u79ef\u7684\u6c34\u6d3b\u5316\uff0c\u6d3b\u5316\u540e\u7684\u56fa\u76f8\u8403\u53d6\uff08SPE\uff09\u67f1\u586b\u6599\u52a02\u500d\u4f53\u79ef\u7684\u6c34\u7f6e\u4e8e2\uff5e8\u2103\u51b0\u67dc\u4e2d\u907f\u5149\u4fdd\u5b58\u3002
\u5c06\u666e\u901a\u5b9a\u6027\u6ee4\u7eb8\u526a\u6210\u4e0e2.5\uff4d\uff2c\u6ce8\u5c04\u5668\u5185\u5f84\u5927\u5c0f\u76f8\u540c\u7684\u57ab\u7247\u7f6e\u4e8e\u6ce8\u5c04\u5668\u5e95\u90e8\uff0c\u52a0\u5c11\u91cf\u6c34\u62bd\u5e72\uff0c\u4f7f\u5176\u4e0e\u6ce8\u5c04\u5668\u5e95\u90e8\u7d27\u5bc6\u8d34\u5408\uff0c\u79fb\u53d6\u6d3b\u5316\u597d\u7684NM100-SSCX\u5f3a\u9178\u6027\u9633\u79bb\u5b50\u4ea4\u6362\u6811\u8102\u586b\u6599\u586b\u5165\u6ce8\u5c04\u5668\u4e2d\uff0c\u6bcf\u4e2a\u67f1\u7ba1\u5185\u586b\u51450.5\uff4d\uff2c\u5f85\u7528\uff08\u586b\u6599\u8981\u5747\u5300\u586b\u5145\uff0c\u9632\u6b62\u6c14\u6ce1\u4ea7\u751f\uff0c\u4e0a\u9762\u8981\u4fdd\u7559\u5c11\u91cf\u6c34\uff0c\u4e0d\u8981\u6d41\u5e72\uff09\u3002
\u79fb\u53d6\u6837\u54c1\u63d0\u53d6\u6db25\uff4d\uff2c\u8fc7SPE\u67f1\uff08\u6ce8\u610f\u63a7\u5236\u6d41\u91cf\uff09\uff0c\u7528\u6c34\u6dcb\u6d17\u81f3\u6dcb\u6d17\u6db2\u5448\u4e2d\u6027\uff0c\u52a05mol\u00b7L\uff0d1<\/sup>\u6c2f\u5316\u94f5\u6eb6\u6db215mL\u6d17\u8131\uff0c\u6536\u96c6\u6d17\u8131\u6db2\u4e8e50mL\u79bb\u5fc3\u7ba1\u4e2d\u3002<\/span><\/p>\n

1.3.3 \u884d\u751f<\/span><\/p>\n

\u5411\u79bb\u5fc3\u7ba1\u4e2d\u52a0\u516512mol\u00b7L\uff0d1<\/sup>\u00a0\u6c22\u6c27\u5316\u94a0\u6eb6\u6db212\uff4d\uff2c\uff0c10\uff47\u00b7\uff2c\uff0d1<\/sup>\u94c1\u6c30\u5316\u94be\u6eb6\u6db21\uff4d\uff2c\uff0c\u6447\u5300\uff0c\u5f85\u79bb\u5fc3\u7ba1\u4e2d\u6eb6\u6db2\u51b7\u5374\u81f3\u5ba4\u6e29\u540e\uff0c\u52a0\u5165\u4e8c\u6c2f\u7532\u70f720\uff4d\uff2c\uff0c\u5267\u70c8\u632f\u64470.5min\uff0c\u4ee5\u8f6c\u901f3500\uff52\u00b7min\uff0d1<\/sup>\u79bb\u5fc31min\uff0c\u79fb\u53d6\u4e0b\u5c42\u6eb6\u6db2\u81f3150mL\u65cb\u84b8\u74f6\u4e2d\uff0c\u518d\u52a0\u5165\u4e8c\u6c2f\u7532\u70f720mL\u91cd\u590d\u63d0\u53d6\u4e00\u6b21\uff0c\u5408\u5e76\u4e8c\u6c2f\u7532\u70f7\u5c42\uff0c\u4e8e40\u2103\u6c34\u6d74\u4e2d\u51cf\u538b\u65cb\u84b8\u81f3\u5e72\u3002\u7528\u4e59\u8148\uff08\uff11\uff0b\uff19\uff09\u6eb6\u6db22\uff4d\uff2c\u8d85\u58f0\u6eb6\u89e3\u6b8b\u6e23\u540e\uff0c\u8fc70.22\u03bc\uff4d\u6709\u673a\u7cfb\u6ee4\u819c\uff0c\u6309\u4eea\u5668\u5de5\u4f5c\u6761\u4ef6\u8fdb\u884c\u6d4b\u5b9a\u3002<\/span><\/p>\n

1.3.4 0.1\uff4d\uff47\u00b7\uff2c\uff0d1<\/sup>\u767e\u8349\u67af\u884d\u751f\u7269\u6807\u51c6\u6eb6\u6db2\u7684\u5236\u5907\u79fb\u53d62.0\uff4d\uff47\u00b7\uff2c\uff0d1<\/sup>\u767e\u8349\u67af\u6807\u51c6\u6eb6\u6db20.5\uff4d\uff2c\u4e8e\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u52a0\u51655mol\u00b7L\uff0d1<\/sup>\u6c2f\u5316\u94f5\u6eb6\u6db215\uff4d\uff2c\uff0c\u5f85\u79bb\u5fc3\u7ba1\u4e2d\u6eb6\u6db2\u51b7\u5374\u81f3\u5ba4\u6e29\uff0c\u52a0\u5165\u4e8c\u6c2f\u7532\u70f720\uff4d\uff2c\u5267\u70c8\u632f\u64470.5min\uff0c\u4ee5\u8f6c\u901f3500\uff52\u00b7min\uff0d1<\/sup>\u79bb\u5fc31min\uff0c\u79fb\u53d6\u4e0b\u5c42\u6eb6\u6db2\u81f3150\uff4d\uff2c\u65cb\u84b8\u74f6\u4e2d\uff0c\u518d\u52a0\u5165\u4e8c\u6c2f\u7532\u70f720\uff4d\uff2c\u91cd\u590d\u63d0\u53d6\u4e00\u6b21\uff0c\u5408\u5e76\u4e8c\u6c2f\u7532\u70f7\u5c42\uff0c\u4e8e40\u2103\u6c34\u6d74\u4e2d\u51cf\u538b\u65cb\u84b8\u81f3\u5e72\u3002\u7528\u4e59\u8148\uff08\uff11\uff0b\uff19\uff09\u6eb6\u6db210\uff4d\uff2c\u8d85\u58f0\u6eb6\u89e3\u6b8b\u6e23\u540e\uff0c\u8fc70.22\u03bc\uff4d\u6709\u673a\u7cfb\u6ee4\u819c\uff0c\u8f6c\u5165\u68d5\u8272\u50a8\u6db2\u74f6\u4e2d\uff0c\u4e8e4\u2103\u51b7\u85cf\u4fdd\u5b58\uff0c\u6709\u6548\u671f\u4e3a\u4e00\u4e2a\u6708\u3002\u8be5\u884d\u751f\u7269\u6807\u51c6\u6eb6\u6db2\u6027\u8d28\u7a33\u5b9a\uff0c\u6bcf\u6b21\u4f7f\u7528\u65f6\uff0c\u53ef\u4ee5\u76f4\u63a5\u7a00\u91ca\u81f3\u6240\u9700\u8d28\u91cf\u6d53\u5ea6\u3002<\/span><\/p>\n

2 \u7ed3\u679c\u4e0e\u8ba8\u8bba<\/strong><\/span><\/span><\/p>\n

2.1 \u8272\u8c31\u884c\u4e3a<\/strong><\/span><\/p>\n

\u7eff\u8336\u6837\u54c1\u4e2d\u6dfb\u52a010\u03bc\uff47\u00b7kg\uff0d1<\/sup>\u00a0\u767e\u8349\u67af\u6807\u51c6\u6eb6\u6db2\uff0c\u5176MRM \u8272\u8c31\u56fe\u89c1\u56fe\uff11\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\"\"<\/span><\/p>\n

2.2 \u8272\u8c31\u6761\u4ef6\u7684\u9009\u62e9<\/strong><\/span><\/p>\n

2.2.1 \u6d41\u52a8\u76f8<\/span><\/p>\n

\u8bd5\u9a8c\u5206\u522b\u8003\u5bdf\u4e86\u7532\u9187\u4f53\u7cfb\u548c\u4e59\u8148\u4f53\u7cfb\u4f5c\u4e3a\u6d41\u52a8\u76f8\u65f6\u5bf9\u6d4b\u5b9a\u7684\u5f71\u54cd\u3002\u7ed3\u679c\u8868\u660e\uff1a\u8fd9\u4e24\u79cd\u4f53\u7cfb\u4f5c\u4e3a\u6d41\u52a8\u76f8\u65f6\uff0c\u4eea\u5668\u7684\u7075\u654f\u5ea6\u5728\u540c\u4e00\u6c34\u5e73\u4e0a\u3002\u8003\u8651\u5230\u4f7f\u7528\u7532\u9187\u4f53\u7cfb\u7cfb\u7edf\u538b\u529b\u9ad8\uff0c\u8bd5\u9a8c\u9009\u62e9\u4e59\u8148\u4f53\u7cfb\u4f5c\u4e3a\u6d41\u52a8\u76f8\u3002<\/span><\/p>\n

2.2.2 \u8272\u8c31\u67f1<\/span><\/p>\n

\u767e\u8349\u67af\u6781\u6027\u5f88\u5f3a\uff0c\u91c7\u7528Water BEH UPLC C18<\/sub>\u8272\u8c31\u67f1\u65e0\u6cd5\u5f97\u5230\u4fdd\u7559\uff0c\u4e00\u822c\u91c7\u7528Hillic\u8272\u8c31\u67f1\u8fdb\u884c\u5206\u79bb\uff0c\u4e3a\u4e86\u83b7\u5f97\u826f\u597d\u7684\u5cf0\u5f62\uff0c\u6d41\u52a8\u76f8\u4e2d\u5fc5\u987b\u6dfb\u52a0\u9ad8\u542b\u91cf\u7684\u76d0\uff0c\u4f46\u9ad8\u542b\u91cf\u7684\u76d0\u5bb9\u6613\u9020\u6210\u4eea\u5668\u6c61\u67d3\u3002\u8bd5\u9a8c\u5c06\u76ee\u6807\u7269\u8fdb\u884c\u4e86\u884d\u751f\u53cd\u5e94\uff0c\u5176\u884d\u751f\u53cd\u5e94\u4ea7\u7269\u5728Waters BEH UPLC C18<\/sub>\u8272\u8c31\u67f1\u4e0a\u83b7\u5f97\u4e86\u5f88\u597d\u7684\u5206\u79bb\u6548\u679c\u3002\u8bd5\u9a8c\u9009\u7528Waters BEH UPLC C18<\/sub>\u8272\u8c31\u67f1\u3002<\/span><\/p>\n

2.3 \u8d28\u8c31\u6761\u4ef6\u7684\u9009\u62e9<\/strong><\/span><\/p>\n

2.3.1 \u6bcd\u79bb\u5b50<\/span><\/p>\n

\u767e\u8349\u67af\u5728\u78b1\u6027\u6761\u4ef6\u4e0b\u88ab\u94c1\u6c30\u5316\u94be\u6c27\u5316\u62101\uff0c1\uff0d\u4e8c\u7532\u57fa\uff0d\uff3b4\uff0c4\u2032\uff0d\u8054\u5421\u5576\uff3d\uff0d2\uff0c2\u2032\uff0d\u4e8c\u916e\uff0c\u767e\u8349\u67af\u7684\u884d\u751f\u53cd\u5e94\u5f0f\u89c1\u56fe\uff12\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u6839\u636e\u767e\u8349\u67af\u7684\u7ed3\u6784\uff0c\u63a8\u65ad\u5176\u6bcd\u79bb\u5b50\u788e\u7247m\uff0fz\u00a0\u5e94\u8be5\u662f217\uff0c\u6b64\u63a8\u65ad\u4e5f\u5728\u8d28\u8c31\u5206\u6790\u4e2d\u5f97\u5230\u4e86\u5f88\u597d\u7684\u9a8c\u8bc1\u3002\u8bd5\u9a8c\u9009\u62e9\u767e\u8349\u67af\u884d\u751f\u7269\u7684\u6bcd\u79bb\u5b50\uff4d\uff0f\uff5a \u4e3a217\u3002<\/span><\/p>\n

2.3.2 \u5b50\u79bb\u5b50<\/span><\/p>\n

\u56e0\u884d\u751f\u53cd\u5e94\u5f97\u5230\u7684\u65b0\u7269\u8d28\u6240\u5728\u7684\u57fa\u8d28\u73af\u5883\u590d\u6742\uff0c\u4e3a\u83b7\u5f97\u597d\u7684\u8d28\u8c31\u56fe\uff0c\u8bd5\u9a8c\u9700\u8981\u5148\u901a\u8fc7\u8272\u8c31\u67f1\u5c06\u65b0\u7269\u8d28\u4e0e\u6742\u8d28\u5206\u79bb\uff0c\u7136\u540e\u518d\u5bf9\u5176\u8fdb\u884c\u8d28\u8c31\u65b9\u6cd5\u7684\u4f18\u5316\u3002
\u767e\u8349\u67af\u5b50\u79bb\u5b50\u7684\u626b\u63cf\u8d28\u8c31\u56fe\u89c1\u56fe\uff13\u3002
\u7531\u56fe\uff13\u53ef\u77e5\uff1a174\uff0c160\uff0c158\uff0c148\uff0c130\uff0c117\u90fd\u662f\u788e\u7247\u79bb\u5b50\uff0c\u5176\u88c2\u89e3\u539f\u7406\u89c1\u56fe4\u3002\u518d\u901a\u8fc7\u7cbe\u786e\u4f18\u5316\u786e\u5b9a\u5404\u5b50\u79bb\u5b50\u7684\u6700\u4f73\u8d28\u8c31\u6761\u4ef6\uff0c\u4ee5\u4fbf\u83b7\u5f97\u6700\u4f73\u54cd\u5e94\u548c\u7cbe\u786e\u8d28\u91cf\u6570\uff0c\u8bd5\u9a8c\u9009\u62e9\uff4d\uff0f\uff5a 174.23\u4f5c\u4e3a\u5b9a\u91cf\u79bb\u5b50\uff0c\uff4d\uff0f\uff5a 148.24\u4f5c\u4e3a\u5b9a\u6027\u79bb\u5b50\u3002<\/span><\/p>\n

2.4 \u6807\u51c6\u66f2\u7ebf\u3001\u68c0\u51fa\u9650\u548c\u6d4b\u5b9a\u4e0b\u9650<\/strong><\/span><\/p>\n

\"\"<\/span><\/p>\n

\u6309\u8bd5\u9a8c\u65b9\u6cd5\u5bf9\u767e\u8349\u67af\u884d\u751f\u7269\u6807\u51c6\u6eb6\u6db2\u7cfb\u5217\u8fdb\u884c\u6d4b\u5b9a\uff0c\u5e76\u7ed8\u5236\u6807\u51c6\u66f2\u7ebf\u3002\u7ed3\u679c\u8868\u660e\uff1a\u767e\u8349\u67af\u7684\u8d28\u91cf\u6d53\u5ea6\u57280.001\uff5e0.1\uff4d\uff47\u00b7\uff2c\uff0d\uff11<\/sup>\u5185\u4e0e\u5176\u5bf9\u5e94\u7684\u5cf0\u9762\u79ef\u5448\u7ebf\u6027\u5173\u7cfb\uff0c\u7ebf\u6027\u56de\u5f52\u65b9\u7a0b\u4e3a\uff59\uff1d2.100\u00d7106<\/sup>\uff58<\/span>-3.242\u00d7102<\/sup>\uff0c\u76f8\u5173\u7cfb\u6570\u4e3a0.9998\u3002
\u4ee53\u500d\u4fe1\u566a\u6bd4\u8ba1\u7b97\u767e\u8349\u67af\u7684\u68c0\u51fa\u9650\uff083S\uff0fN\uff09\u4e3a0.5\u03bc\uff47\u00b7\uff2c\uff0d\uff11<\/sup>\uff0c\u4ee510\u500d\u4fe1\u566a\u6bd4\u8ba1\u7b97\u767e\u8349\u67af\u7684\u6d4b\u5b9a\u4e0b\u9650\uff0810\uff33\uff0f\uff2e\uff09\u4e3a0.01\uff4d\uff47\u00b7\uff4b\uff47\uff0d\uff11<\/sup>\u3002<\/span><\/p>\n

2.5 \u65b9\u6cd5\u7684\u91cd\u590d\u6027\u548c\u51c6\u786e\u6027<\/strong><\/span><\/p>\n

\u6309\u8bd5\u9a8c\u65b9\u6cd5\u5bf9\u7eff\u8336\u6837\u54c1\u8fdb\u884c\u5206\u6790\uff0c\u5e76\u8fdb\u884c\u52a0\u6807\u56de\u6536\u8bd5\u9a8c\uff0c\u8ba1\u7b97\u6d4b\u5b9a\u603b\u91cf\u7684\u76f8\u5bf9\u6807\u51c6\u504f\u5dee\uff08RSD\uff09\u548c\u52a0\u6807\u56de\u6536\u7387\uff0c\u7ed3\u679c\u89c1\u8868\uff12\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

2.6 \u5b9e\u9a8c\u5ba4\u95f4\u6570\u636e\u6bd4\u8f83<\/strong><\/span><\/p>\n

\u4e3a\u9a8c\u8bc1\u65b9\u6cd5\u7684\u51c6\u786e\u6027\u548c\u53ef\u9760\u6027\uff0c\u8bd5\u9a8c\u5c06\u5df2\u7ecf\u7c89\u788e\u597d\u7684700\uff47\u7eff\u8336\u6837\u54c1\u4f5c\u4e3a\u6837\u54c1\uff11\uff0c\u5e76\u5236\u5907\u4e86\u4e00\u4e2a\u767e\u8349\u67af\u7406\u8bba\u503c\u4e3a0.2\uff4d\uff47\u00b7kg\uff0d1<\/sup>\u7684\u9633\u6027\u7eff\u8336\u6837\u54c1\uff08\u6837\u54c12\uff09\u3002<\/span><\/p>\n

\u7528\u6837\u54c11\u3001\u6837\u54c12\u8fdb\u884c\u4e86\u5b9e\u9a8c\u5ba4\u95f4\u7684\u6bd4\u5bf9\uff0c\u5176\u7ed3\u679c\u89c1\u88683\u3002<\/span><\/p>\n

\"\"<\/span><\/p>\n

\u6837\u54c1\u82e5\u4e0d\u7ecf\u8fc7\u6d78\u6ce1\u548c\u78fa\u5316\u5904\u7406\uff0c\u76f4\u63a5\u7528\u6c34\u63d0\u53d6\u540e\u5206\u6790\uff0c\u7ed3\u679c\u662f\u9634\u6027\uff0c\u518d\u6b21\u8bc1\u660e\u4e86\u672c\u65b9\u6cd5\u7684\u53ef\u9760\u6027\uff0c\u8bf4\u660e\u767e\u8349\u67af\u6613\u949d\u5316\uff0c\u76f4\u63a5\u7528\u6c34\u63d0\u53d6\u5f88\u56f0\u96be\u3002<\/span><\/p>\n

2.7 \u6837\u54c1\u5206\u6790<\/strong><\/span><\/p>\n

\u6309\u8bd5\u9a8c\u65b9\u6cd5\u5bf984\u4e2a\u8336\u53f6\u6837\u54c1\u8fdb\u884c\u5206\u6790\uff0c\u5176\u4e2d\u4e4c\u9f99\u833639\u4e2a\uff0c\u8309\u8389\u82b1\u833616\u4e2a\uff0c\u7eff\u83366\u4e2a\uff0c\u7ea2\u83366\u4e2a\uff0c\u7ea2\u8336\u63d0\u53d6\u72694\u4e2a\uff0c\u9ed1\u83366\u4e2a\uff0c\u8336\u63d0\u53d6\u72694\u4e2a\uff0c\u8336\u6d53\u7f29\u6c413\u4e2a\u3002\u7ed3\u679c\u8868\u660e\uff1a\u767e\u8349\u67af\u6d4b\u5b9a\u503c\u9ad8\u4e8e0.01\uff4d\uff47\u00b7kg-1<\/sup>\u7684\u6837\u54c1\u670929\u4e2a\uff0c\u5360\u603b\u6837\u54c1\u6570\u768434.5\uff05\uff0c\u767e\u8349\u67af\u6d4b\u5b9a\u503c\u6700\u9ad8\u4e3a0.22mg\u00b7kg-1<\/sup>\uff0c\u767e\u8349\u67af\u6d4b\u5b9a\u503c\u4e3a0.01\uff5e0.05\uff4d\uff47\u00b7\uff4b\uff47-1<\/sup>\u7684\u6837\u54c1\u5360\u603b\u9633\u6027\u7ed3\u679c\u768482.7\uff05\u3002
\u672c\u5de5\u4f5c\u91c7\u7528\u8d85\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff0d\u4e32\u8054\u8d28\u8c31\u6cd5\u6d4b\u5b9a\u8336\u53f6\u4e2d\u767e\u8349\u67af\u7684\u542b\u91cf\u3002\u672c\u65b9\u6cd5\u5177\u6709\u63d0\u53d6\u6548\u7387\u5145\u5206\u3001\u51c6\u786e\u5ea6\u597d\u3001\u7075\u654f\u5ea6\u9ad8\u7b49\u7279\u70b9\uff0c\u53ef\u6ee1\u8db3\u8336\u53f6\u7c7b\u590d\u6742\u57fa\u8d28\u7684\u68c0\u6d4b\u8981\u6c42\u3002<\/span><\/p>\n

\u53c2\u8003\u6587\u732e<\/strong><\/span><\/span><\/p>\n

[1] \u8521\u6625\u5e73,\u7fc1\u82e5\u8363,\u6881\u9e23,\u7b49.AOCO\u6d4b\u5b9a\u519c\u836f\u767e\u8349\u67af\u542b\u91cf\u65b9\u6cd5\u7684\u4e0d\u8db3\u548c\u6539\u8fdb[J].\u68c0\u9a8c\u68c0\u75ab\u79d1\u5b66,2004,14(3):15-17.
[2] CHEN J G,ELDRIDGE D L,LODESERTO F J,etal.Paraquat ingestion:A challenging diagnosis[J].Pediatrics, 2010,125(6):505-509.
[3] ALMEIDA R M D,YONAMINE M.Gas chromatographic-mass spectrometric method for the determination of the herbicides paraquat and diquat in plasma and urine samples[J].Journal of Chromatography B, 2007,853(7):260-264.
[4] \u674e\u4fca,\u738b\u9707,\u90ed\u6653\u5173,\u7b49.\u6d4b\u5b9a\u9c7c\u8165\u8349\u4e2d\u7684\u767e\u8349\u67af\u548c\u4e59\u8349\u80fa\u6b8b\u7559\u7684\u4e09\u91cd\u56db\u7ea7\u6746\u6c14\u76f8\u8272\u8c31\u8d28\u8c31\u6cd5[J].\u5c71\u5730\u519c\u4e1a\u751f\u7269\u5b66\u62a5,2012,31(4):341-344.
[5] \u6731\u9707\u6d77,\u5ba3\u680b\u6881.\u56fa\u76f8\u8403\u53d6-HPLC\u6cd5\u6d4b\u5b9a\u6c34\u4e2d\u654c\u8349\u5feb\uff64\u767e\u8349\u67af[J].\u4e2d\u56fd\u536b\u751f\u68c0\u9a8c\u6742\u5fd7,2011,21(9):2154-2156.
[6] \u5b63\u5174\u7e41,\u90b1\u76f8\u541b,\u738b\u52c7,\u7b49.HPLC\u6cd5\u68c0\u6d4b\u4eba\u8840\u6d46\u4e2d\u767e\u8349\u67af\u7684\u6d53\u5ea6[J].\u4e2d\u56fd\u836f\u5e08,2012,15(2):209-211.
[7] \u674e\u5f3a,\u5218\u6d77\u82f1,\u738b\u7389\u7ea2,\u7b49.\u767e\u8349\u67af\u4e2d\u6bd2\u60a3\u8005\u514d\u75ab\u529f\u80fd\u6539\u53d8\u53ca\u4e34\u5e8a\u610f\u4e49[J].\u65b0\u4e61\u533b\u5b66\u9662\u5b66\u62a5,2006,23(6):575-576.
[8] \u8584\u6d77\u6ce2.\u6db2\u76f8\u8272\u8c31-\u4e32\u8054\u8d28\u8c31\u6cd5\u5feb\u901f\u6d4b\u5b9a\u690d\u7269\u6e90\u6027\u98df\u54c1\u4e2d\u7684\u767e\u8349\u67af[J].\u8272\u8c31,2011,29(2):180-183.
[9] \u5218\u840d,\u90ac\u6625\u534e,\u90d1\u529b\u884c,\u7b49.\u751f\u7269\u6837\u54c1\u4e2d\u767e\u8349\u67af\u68c0\u6d4b\u65b9\u6cd5\u7814\u7a76\u8fdb\u5c55[J].\u73af\u5883\u4e0e\u804c\u4e1a\u533b\u5b66,2010(9):563-565.
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[11] PIZZUTTI I R,VELA G M E,KOK A D,et al.Determination of paraquat and diquat:LC-MS method optimization and validation[J].Food Chemistry, 2016,209(15):248-255.<\/span><\/p>\n

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